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pCN39
pCN39
規(guī)格:
貨期:
編號:B238960
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCN39
商品貨號 B238960
Designations pCN39
Depositors R Diaz Orejas
Biosafety Level 1
Vector Information
Size (kb): 4.0999999046325680
Vector: pCN39 (plasmid)
Construction: pUC7, pCN38 (ATCC 77095)
Marker(s):kanR
Construct size (kb): 4.099999904632568
Features: marker(s): kanR
replicon: repA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; KpnI/PvuII--2.6, 1.6; HindIII/PstI--2.8, 1.4.
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
General purpose vector.
Constructed by inserting a 0.9 kb PstI fragment from R1 into the PstI site of pUC7, cloning a 0.9 kb BamHI fragment from this intermediate into the BamHI site of pCN38 (ATCC 77095), and deleting a 0.9 kb PstI fragment,
to generate the equivalent of pCN38 with part of the multiple cloning site of pUC7.
The order of the major features in this plasmid is: PvuII - MCS - kanR - HindIII - KpnI - repA.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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