一级特黄aaa大片在线观看,国产看免费视频,一级毛片,免费无码婬片aaa直播,久久久久国产一区二区三区,性色生活片久久无少妇一级婬片免费放,国产寡妇高潮一级毛片免费看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pRS169
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pRS169
pRS169
規(guī)格:
貨期:
編號:B238728
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pRS169
商品貨號 B238728
Designations pRS169
Depositors P Hieter
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.8000001907348630
DESCRIPTION OF VECTOR:
Intact vector size: 5.800
Type of vector: phagemid
Cloning sites: XhoI SalI EcoRI SmaI BamHI NotI SacI
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI EagI NotI BstXI SacII SacI
Construction: pRSS56 [pBluescript KS+, pBS(+)], GAL1/10 promoter
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac, T3, T7, GAL1
replicon: pMB1, f1, ARS1
Vector: pRS169 (phagemid)
Promoters: Promoter T3
Construction: pRSS56 [pBluescript KS+, pBS(+)], GAL1/10 promoter
Marker(s):URA3,ampR
Construct size (kb): 5.800000190734863
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac, T3, T7, GAL1
replicon: pMB1, f1, ARS1
Applications
YC-type (centromeric) shuttle vector
YX-type (expression) shuttle vector
expression vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--2.9 (doublet); BamHI--5.8; EcoRI--5.8; EcoRV--4.5, 1.3; HindIII--5.8; SalI--5.8; SmaI--5.8; XbaI--uncut.
Expression is inducible by galactose.
YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ') peptide.
Transcription from the GAL1 promoter (no ATG included in the sequence) proceeds through the polylinker.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
A fragment containing the URA3 gene was inserted into the NdeI site and a DraIII/EcoRI fragment containing the GAL1 and GAL10 promoters was inserted into the Asp718 site of the MCS of pRSS56. All ends were blunted.
The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - GAL1 promoter - GAL10 promoter - pMB1 ori - bla - CEN - ARS1.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Philip Hieter, personal communication

Related Products
component of:ATCC 87054
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
闽侯县| 阿克| 洛川县| 漠河县| 神农架林区| 瑞丽市| 余干县| 藁城市| 乐清市| 区。| 珠海市| 冷水江市| 梅河口市| 黑山县| 仁布县| 兴国县| 鱼台县| 上虞市| 资中县| 涿州市| 桃源县| 宁南县| 页游| 奎屯市| 黄骅市| 三台县| 漠河县| 潮安县| 蒲城县| 凤阳县| 黄平县| 剑阁县| 大宁县| 南澳县| 阿图什市| 读书| 阳春市| 金坛市| 麟游县| 克什克腾旗| 连江县|