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Size (kb): 5.7760000228881840

Vector: pXC33b (plasmid)

Promoters: Promoter for expression lambda PL

Construction: pXC24, oligo cassette

Marker(s):ampR

Construct size (kb): 5.776000022888184

Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' PacI site
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: ATTAATTA
transcription terminator: partially deleted lambda terminator
translational enhancer: T7 gene 10
coding sequence: 14-3-3

expression vector

in another host, produces protein

vector useful for cloning PCR products

Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.

The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.

One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.

Temperature: 37.0°C