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pJS97
pJS97
規(guī)格:
貨期:
編號(hào):B234346
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pJS97
商品貨號(hào) B234346
Designations pJS97
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 7.3000001907348630
Vector: pJS97 (YAC)
Promoters: Promoter T7 (phi10)
Marker(s):URA3,ampR,SUP11
Construct size (kb): 7.300000190734863
Features: marker(s): ampR, URA3, SUP11
promoter: T7 (phi10)
replicon: pMB1, ARSH4
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
shuttle vector
vector for constructing genomic libraries
vector for other uses
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): NotI--7.4; BglII--7.4; SalI--7.4; EcoRI--7.4; SacI--7.4.
pJS97 (ATCC 77153) and pJS98 (ATCC 77154) are used together to construct artificial chromosomes. Each plasmid is digested with ClaI and the cloning enzyme.
To recover both ends of a YAC insert as plasmid, DNA of a YAC-containing strain is digested to completion with BamHI, SphI or SacI, diluted, ligated, and transformed into E. coli (to ampicillin resistance).
The insert of a resultant plasmid (which includes the T7 promoter) extends from an end of the YAC insert to the first site of the enzyme used for digestion.
YL-type shuttle vector useful for construction of genomic libraries. Contains promoters for in vitro RNA synthesis and permits recovery of end fragments as plasmids in E. coli.
The order of the major features in this plasmid is: ClaI/MCS/EcoRI - T7 promoter - ARSH4 - CEN4 - URA3 - SUP11 - pMB1 ori - bla - SacI - BamHI - SphI - TEL.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Shero JH, et al. Yeast artificial chromosome vectors for efficient clone manipulation and mapping. Genomics 10: 505-508, 1991. PubMed: 2071158

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