一级特黄aaa大片在线观看,国产看免费视频,一级毛片,免费无码婬片aaa直播,久久久久国产一区二区三区,性色生活片久久无少妇一级婬片免费放,国产寡妇高潮一级毛片免费看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Leishmania infantum Nicolle
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Leishmania infantum Nicolle
Leishmania infantum Nicolle
規(guī)格:
貨期:
編號(hào):B232856
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Leishmania infantum Nicolle
商品貨號(hào) B232856
Strain Designations LIVT-2
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
popliteal lymph node of a foxhound, Virginia
Product Format frozen
Storage Conditions Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic Axenic
Type Strain no
Medium ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions
Temperature: 25°C
Cryopreservation
  1. Harvest cells from cultures that are at or near peak density. Aseptically transfer the broth overlay to a plastic centrifuge tube and adjust the concentration of cells to 2 x 107/ml in fresh medium (broth overlay). If necessary, cells may be concentrated by centrifugation at 800 x g for 5 min.
  2. Prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  Cool on ice.
  3. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO solution to the start of the freezing process should be no less than 15 min and no longer than 30 min.
  4. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  5. Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at        -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  6. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -70°C.
  7. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  8. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing ATCC medium 807.
  9. Incubate the culture vertically at 25°C. Observe the culture daily and transfer when numerous trophozoites are observed.
Name of Depositor DS Lindsay
Special Collection NCRR Contract
References

David S Lindsay, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
南宫市| 新平| 同德县| 新乡市| 西昌市| 琼中| 商南县| 民县| 霍山县| 四川省| 恩平市| 嘉黎县| 林芝县| 乌兰县| 铁力市| 左贡县| 苗栗市| 宿州市| 兴义市| 宽城| 玉溪市| 沈丘县| 成安县| 江华| 镇沅| 新昌县| 寿宁县| 海盐县| 广汉市| 武宁县| 进贤县| 平乐县| 柳江县| 茶陵县| 内江市| 定安县| 岳普湖县| 和平区| 广汉市| 志丹县| 深水埗区|