Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9; BamHI--3.4, 0.5; KpnI--3.1, 0.8. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.42; BglII--0.39; ClaI--0.45; KpnI--0.11; PstI--0.17, 0.80; SphI--0.12. This clone contains 15 bp of the 5'UTR, complete coding sequence, and 265 bp of 3'UTR. |