| 產(chǎn)品名稱 |
Simian virus 40 |
| 商品貨號(hào) |
B224049 |
| Classification |
Polyomaviridae, Polyomavirus |
| Agent |
Simian virus 40 |
| Strain |
pUCSV40-B1E [molecular clone of Simian virus 40, SV-40] |
| Common Name |
Simian virus 40 |
| Applications |
contains sequence |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Contains a full-length genome of SV40-B1 cloned into pUC-19 at the EcoRI site. SV40-B1 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. The viral regulatory region is archetypal. |
| Product Format |
freeze-dried |
| Comments |
This variant of SV40-Baylor has an archetypal viral regulatory region and can be used in viral growth studies for comparison with SV40-B2, the commonly used laboratory variant of SV40-Baylor. Contains a full-length genome of SV40-B1 cloned into pUC-19 at the EcoRI site. SV40-B1 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. The viral regulatory region is archetypal. |
| Effect on Host |
This variant of SV40-Baylor has an archetypal viral regulatory region and can be used in viral growth studies for comparison with SV40-B2, the commonly used laboratory variant of SV40-Baylor. |
| Growth Medium |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Recommended Host |
E. coli |
| Growth Conditions |
Temperature: 37°C |
| Name of Depositor |
JA Lednicky, JS Butel |
| Special Collection |
NCRR Contract |
| References |
Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047
Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955
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