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Size (kb): 5.0710000991821290

Vector: pKSM710 (phagemid)

Promoters: Promoter for expression lambda PL

Construction: pSM702, pMAMPF3

Marker(s):ampR,cmlR (am)

Construct size (kb): 5.071000099182129

Features: insert detection: lacZalpha
marker(s): ampR
marker(s): cmlR (am)
promoter for expression: lacUV5
promoter for expression: lambda PL
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB

contains easily purifiable cassette(s) for construction

expression vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57.

Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors.

Temperature: 37.0°C