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pDG3
pDG3
規(guī)格:
貨期:
編號:B219505
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDG3
商品貨號 B219505
Designations pDG3
Depositors Allelix Biopharmaceuticals, Inc., G Strachan, Allelix Biopharmaceuticals, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Escherichia coli JM83; K-12 strain JM83
Vector Information
Size (kb): 7.8000001907348630
Vector: pDG3 (plasmid)
Construction: pUC12, Tn5, pBB116
Marker(s):ampR,neoR,argB
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, neoR, argB
replicon: pMB1
Applications
integrating vector
shuttle vector
vector permitting positive selection for integration
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/SalI--3.1, 1.65, 1.3, 0.75, 0.5 + smaller; XbaI--4.4, 3.4; EcoRI--7.8; SmaI--4.3, 3.5; SphI--7.8.
This is an integrative, selectable vector conferring arginine prototrophy on arginine autotrophic hosts such as Aspergillus niger 350(-)52, ATCC 20739 (argB-). It also confers kanamycin resistance.
This was constructed by inserting a HindIII/SalI fragment of Tn5 into pUC12 cut with HindIII/SalI (pNE02) and then inserting an XbaI fragment from pBB116 into the XbaI site of pNE02.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Buxton FP, et al. Aspergillus niger transformation system. US Patent 4,885,249 dated Dec 5 1989

Berse B, et al. Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene 25: 109-117, 1983. PubMed: 6363209

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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