一级特黄aaa大片在线观看,国产看免费视频,一级毛片,免费无码婬片aaa直播,久久久久国产一区二区三区,性色生活片久久无少妇一级婬片免费放,国产寡妇高潮一级毛片免费看

Size (kb): 2.7000000476837160

Vector: pUC13 (plasmid)

Promoters: Promoter lac

Construction: pBR322, Escherichia coli lacZalpha, M13mp11

Marker(s):ampR

Construct size (kb): 2.700000047683716

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
enhancer: none

cloning vector

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; SalI--2.7; PstI--2.7; XbaI--2.7.

The only difference between this and pUC12 is orientation of polylinker sites.

Temperature: 37.0°C