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in another host, produces protein estrogen receptor

Construct size (kb): 7.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p636
Intact vector size: 5.500
Type of vector: plasmid
Vector end: EcoRV
Vector end: BamHI
Cloning sites: EcoRV, BamHI
Polylinker sites:
Construction: pHyg, CMV promoter, TK promoter/hygR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, hygR
promoter: CMV
replicon: pMB1
terminator: SV40 polyadenylation
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ESR
Genomic copy number: unique
Gene name: estrogen receptor
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 q25.1
Tissue: breast cancer cell line, MCF-7
Type of DNA: cDNA
Insert 5' end: EcoRV
Insert 3' end: BamHI
Insert size (kb): 1.9
Cross references: DNA Seq. Acc.: M12674

Insert lengths(kb): 1.899999976158142

Tissue: breast cancer cell line, MCF-7

Gene product: estrogen receptor [ESR]

Alleles: A1, A2

Target Gene: estrogen receptor

Restriction digests of the clone give the following sizes (kb): BamHI--7.4; ClaI--7.4; HindIII--6.5, 1.3; XbaI--6.4, 1.4; BamHI/EcoRV--7.4; SacI--5.4, 2.1.

Electrophoretic analysis of restriction digests suggests that the EcoRV site has been lost.

Restriction digests of the clone give the following sizes (kb): HindIII--6.1, 1.3; XbaI--6.0, 1.4.

Contains the wild type complete coding sequence. Corresponds to GenBank accession M12674 with a T->G substitution at nt 1491.

There are additional SacI sites in the insert.

Constructed by inserting an 1.9 kb EcoRI fragment from HEGO into pBluescript SK and then transferring the EcoRV/BamHI fragment to p636. The insert therefore contains some contains some Bluescript sequence.

The order of the major features in this plasmid is: ClaI - CMV promoter - SacI - EcoRV - ESR - BamHI - SV40 polyadenylation - HSV-1 tk polyadenylation - <- hgrR - HSV-1 tk promoter - ampR.