Insert |
DNA: genomic Insert lengths(kb): 14.30000019073486 Tissue: B lymphocyte (EBV transformed), SH cell line Gene product: T cell receptor, gamma, variable region V5P (pseudogene) [TCRGV6] |
Comments |
Restriction digests of the clone give the following sizes (kb): KpnI--18.0, 14.0, 5.8, 4.4, 1.8, 1.5; PvuI--23.0, 13.0, 7.9; SacI--20.0, 9.0, 5.6, 4.4, 3.8, 0.9; SmaI--20.0, 9.5, 9.0, 5.8; XhoI--20.0, 15.0, 9.0. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--4.2, 9.7, 13.4, 14.3. Contains the genes in the germ line configuration, beginning about 0.4 kb upstream of pseudogene Vgamma5P and extending about 4 kb beyond pseudogene Vgamma7. Additional GenBank/EMBL accession numbers: M13432, M13433. |
References |
LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743
Marie-Paule Lefranc, personal communication
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