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C7
C7
規(guī)格:
貨期:
編號:B164100
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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甘油
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產(chǎn)品名稱 C7
商品貨號 B164100
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with partially purified bovine adrenal LDL receptor.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor
Comments
Mice were immunized with partially purified bovine adrenal LDL receptor.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JL Goldstein
Deposited As mouse (B cell); mouse (myeloma)
References

Beisiegel U, et al. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256: 11923-11931, 1981. PubMed: 6271765

Schneider WJ, et al. Purification of the low density lipoprotein receptor, an acidic glycoprotein of 164,000 molecular weight. J. Biol. Chem. 257: 2664-2673, 1982. PubMed: 6277909

Beisiegel U, et al. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J. Biol. Chem. 257: 13150-13156, 1982. PubMed: 6290495

Tolleshaug H, et al. Post translational processing of the LDL receptor and its genetic disruption in familial hypercholesterolemia. Cell 30: 715-724, 1982. PubMed: 6291781

Tolleshaug H, et al. The LDL receptor locus in familial hypercholesterolemia: multiple mutations disrupt transport and processing of a membrane receptor. Cell 32: 941-951, 1983. PubMed: 6299582

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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