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1D3
1D3
規(guī)格:
貨期:
編號:B163620
品牌:Mingzhoubio

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產(chǎn)品名稱 1D3
商品貨號 B163620
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD19
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD19
Comments

The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19.

The antibody binds to mouse CD19 with high affinity, but does not react with human CD19.

Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium. Establish new cultures at 2 x 10viable cells/mL and maintain at between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Isotype IgG2a; kappa light chain
Name of Depositor DT Fearon, I Krop, MS Schlissel, AR de Fougerolles
References

Krop I, et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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