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11B11 [BSF-1]
11B11 [BSF-1]
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貨期:
編號:B163545
品牌:Mingzhoubio

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產(chǎn)品名稱 11B11 [BSF-1]
商品貨號 B163545
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified BSF-1 emulsified in complete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Cellular Products
immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
70% Culture Media + 20% FBS + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Isotype IgG1
Name of Depositor WE Paul
Deposited As rat (B cell); mouse (myeloma)
References

Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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